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Milk Ring Test Brucella Antigen
(MRTA)
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CONTENTS :
The antigen is prepared from a concentrated cell suspension of Co2 independent smooth strain of Br. abortus (Strain 99). The cells are stained with haematoxylin and suspended in buffered diluent (pH 4.0) .
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PACKAGE :
Glass bottles of 20 ml to apply 600 test.
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INDICATIONS :
It is used to determine the possible presence of Brucellosis in a herd of cows by the milk ring test (MRT). It has not proved effective with pasteurized or treated milk, also with milk of sheep and goats.
N.B. Herds from which the milk ring test is positive should be examined by serological tests to identify the infected animals.
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DIRECT OF USE :
Collection of milk samples : Milk contained in cans or holding tanks is thooughly mixed. Samples of milk is obtained from each can of milk, but from no more than 3 cans should be pooled in each collection tube (14 x 100 mm.). When collecting milk from holding tanks, milk from no more than one holding tanks should be put in each collection tube.
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METHOD OF PERFORMING THE TEST :
1. Mix the milk thoroughly and transfer on ml to a narrow test (11 x 100 mm.) .
2. Add one drop (0.03 ml) of the antigen and mix.
3. Incubate at 37o C for one hour and read using a uniform light source.
4. If the intensity of blue colour in the cream layer is equal to or deeper than in the skim portion, the test is considered negative.
5. If the intensity of the colour in the cream layer is less than in the skim potion, the test is considered negative.
N.B. False positive may occur at periods where a high proportion of
the animals in the herd are drying off, or where mastitis is
prevalent.
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PRECAUTIONS :
1. Milk samples should be mixed thoroughly before applying the test.
2. Shake the antigen very well before use.
3. Known positive and negative samples should be used in each test.
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STORAGE :
Freezing is avoided, store at 4 – 8 oC
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