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Salmonella Pullorum Tube Antigen:
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COMPOSITION:
It consists of standard strain of Salmonella Pullorum, in phenol saline solution.
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INDICATION:
Used in testing native and foreign breeds for pullorum disease by tube agglutination test. Serum from mature chickens, turkeys, ducks and geese can be tested by this antigen.
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Direction for use:
The antigen is diluted as recommended on the label by alkaline phenol saline (pH 8.2 – 8.5) just before performing the test. The diluted antigen is prepared fresh each day to minimize dissolution and lysis of cells.
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READING OF THE TEST:
Agglutination titre of 1/40 or more will indicate salmonella pullorum infection, but if positive agglutination occurs at 1/20 only, it is considered suspicious and should be repeated after two weeks.
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PRECAUTIONS:
- Freezing should be avoided.
- The bottle is shaken very well before use.
- The antigen is diluted as recommended on the label.
- Alkaline phenol saline (pH 8.2 – 8.5) is used to prevent
the formation of confusing “cloudy” reactions.
- Fresh diluted antigen is used as much as possible.
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STORAGE AND EXPIRATION DATE:
Keep at 4-8oC. Avoid freezing.
The antigen is valid for one year.
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PACKAGE:
Bottles of 20 and 100 ml. of concentrated antigen.
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