..::Veterinary Serum and Vaccine Research Institute::..

Proc. The1 st Intern. Conf. Vet. Res. Div., NRC, Cairo , Egypt , February 15-17 th , 2004

Tetravalent Dog Vaccine (A Vaccine aganist canine distemper, canine parvo, canine hepatitis and rabies)

Attyat, M.K. and A.M. Douad

Veterinary Serum and Vaccine Research Institute,
Abbassia, P.O.B. 131, Cairo , Egypt.

Summary

	In a trial to achieve a highly potent and safe cell culture vaccines against rabies, canine parvo, canine distemper and infectious canine hepatitis, two forms of a combined vaccine were prepared. The first form was composed of lyophilized living attenuated cell culture vaccine containing the protective dose of each of canine parvo, canine distemper and canine adenovirus-1 and dissolved on use in the inactivated rabis vaccine . The 2 nd form was an inactivated tetra-dog vaccine containing the four inactivated viruses and adjuvanted with alhydragel.

	The protective dose of the 2 nd form was depending on the required of viral antigen protein as detected previously for each single virus vaccine, such vaccines were proved to be safe in either mice or dogs. Evaluation of such vaccines were performed in 5-8 month aged dogs vaccinated subcutaneously showing no sign of disease after vaccination or after challenge with the virulent virus strains.

	These animals exhibited good levels of serum neutralizing antiobody titers indicating that there was no antagonizing effect of any virus against the immune response dogs on other virus. Keyword: Canine distemper, canine parvo, infectious canine hepatitis, rabies, dog vaccines and polyvalent vaccines.