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| Production of pigeon pox vaccine from the Egyptian local strain on SPF eggs
Samir, S.S. and Daoud, A.M
Veterinary Serum and Vaccine Research Institute,Abbassia, Cairo. |
| Summary |
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A local virulent Egyptian strain of pigeon pox virus was attenuated on specific pathogen free ( SPF) eggs for production of pigeon pox vaccine. Twenty two serial passages for the virulent virus were applied on the chorioallantoic membtane ( CAM ) of SPF eggs. Titration of the serial passages were recorded as 10 6.8 , 10 7.5 , 10 8.5 and 10 8.5 EID 50 / ml for the 5 th , 10 th , 15 th , 18 th and 20 th passages, respectively.
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The path ogenicity of the 5 th , 10 th , 15 th and 18 th passages were tested by inoculation in susceptible pigeons, the 15 th and 18 th passages produced the most acceptable and mild post vaccinal reaction. The 18 th passage was considered the passage having the highest titer (10 8.5 EID 50 / ml).
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The quality control of the prepared vaccine was applied by testing sterility, sasgety and potency; the vaccine was proved to be sterile, safe and potent for pigeons which resisted the challenge with virulent pigeon pox virus.
Confrimatory serological tests (SNT and ELISA) were applied and recorded that the antibody levels after vaccination reached the protective levels (moer than 1.5 index for SNT and more than 1.0 for ELISA).
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