..::Veterinary Serum and Vaccine Research Institute::..

6 th Vet. Med. Zag. Conference (7-9 Spet. 2002) Hurghada

Production of attenuated sheep pox vaccine from the Egyptian strain

Samir, S.S. olfat E. Nakhla; Manal Awad; Michael, A. and Daoud, A.M.

Veterinary Serum and Vaccine Research Institute,Abbassia, Cairo.

Summary

	The Egyptain strain of sheep pox virus waas purified and adapted on tissue cultures; lamb testicle cells and vero cell line, for 27 passages till reached the clearest cytopathic effect (CPE) after 5 days post inocultion with production of a highest titer of virus ( 10 6.1 TCID 50 /ml).

	For vaccine production the tissue culture adapted virus was added to an equal volum of lactalbumin sucrose stabilizer and lyophilizer; the lyophilized vaccine had a titre equal to 10 5.5 TCID 50 /ml in tissue culture and 10 5 EID 50 / ml in susceptible lambs. The prepared vaccine passed successfully the sterility, safety and potency tests, as well as the duration of protection acquired from vaccination was laso estimated and proved that the vaccinated lambs with prepared Egyptain tissue culture sheep pox vaccine resisted challenge with the virulent sheep pox virus for 10 months post vaccination (period of study).

	The humoral antibody level in the serum of vaccinated lambs was measured by virus neutralization test (VNT) and enzyme linked immuno-sorbent assay (ELISA) and proved that the antiobdies against sheep pox virus could be detected from the 1 st to 4 th week post vaccination and persisted for up to 3 weeks post challenge.