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| J. Egypt Vet. Med. Ass. 63. no 2: 111-117,(2003)
Optimtion of some factors for improving PPR vaccine
Laila,A. Sedeek; Fatma, S.Mohamed; Hussein,A. H.and Daoud, A.M.
Veterinary Serum and Vaccine Research Institute,Abbassia, Cairo.
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Three virus-culture interacting aspects were investigated aiming for producing a PPR virus vaccine with superior quality. It has been found that the use of virus inoculum containing multiplicity of infection (MOI) 5per cell, the utilization of rolling culture system and the inoculation of the virus on cell suspension were the best conditions under which maximum PPR virus titer on Vero cell could be achieved.
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These data were persistently confirmed through the production of three successive PPR pilot batches possessing at least 6.5 Iog 10 TCID 50 / ml on Vero cells. It has been deduced that Vero cells at logarithmic phase are more succeptible to the virus infection than the mature ones.
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Also, the use of (MOI) 5 per cell with the versatile virus while the lower (MOI) per cell with the stationary culture method necessitates more time to get maximumvirus titer during which the cells become older and more reluctant to the virus infection.
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