..::Veterinary Serum and Vaccine Research Institute::..

J. Egypt. Vet. Med . Ass.65,No. 4 : 295-311 (2005)

EFFICACY OF VACCINATION OF EGYPTIAN CATTLE AGAINST BABESIOSIS USING EXOANTIGEN BASED VACCINE

Ashmawy, k.i; Otifi, Y.Z. Amany W. Farah and Mervat, M. Ali

Faculty of veterinary Medicine Alexandria University Egypt.

Veterinary Serum and Vaccine Research Institute,
Abbassia, Cairo, Egypt.

Summary

	The study aimed at evaluating the efficacy of babesia bovis exoantigen vaccine for the prophylkaxis against babesiosis in Egyptran cattle Locally isolated strains of babesia bovis and 24 calves, 12 months old, were used in the study.

	Random amplified polymorphic DNA analysis (RAPD ) generated by polymeras chain rection (PCR) was used PCR identified different polymporphic bands between the isolates indicated genetic variations, The experimental period extended to 12 months.

	The humoral immune response and cell mediated immunity were monitored using indirect fluorescent antibody (IFA),.enczyme linked immuno sorbent assay (ELISA) and lymphocyte blastogensis assay respectively. The results of the newly developed vaccine have been considered promising as it offered both protection and safety as compared to the use of alive vaccine in the immunization of cattle against the disease in Egypt.