..::Veterinary Serum and Vaccine Research Institute::..

J. Egypt. Vet. Med . Ass.65,No 2: 251-260 (2005)

STUDIES ON AVAIN COCCIDIOSIS : PREPARATION AND EVALUATION OF
EIMERIA TENELLA VACCINE IN CHICKENS PRIVATE

Hegazy, N.A.M; Amani, W.Farah; Romany, M.M.; Gamal EL-Din, H.Y. and Hussein, A.Z.

Veterinary Serum and Vaccine Research Institute,
Abbassia, P.O.B. 131, Cairo, Egypt.

Summary

	Attenuated Eimeria tenella vaccine was prepared using 60Co ionizing iradiation. The prepared meterial was experimentally applied in 2 weeks old chicks. Chicks were divided into 4 groups. The 1 st group was vaccinated with a single dose of the vaccine. The 2 nd group was vaccinated with 2 successive doses of the vaccine. The 3 rd and 4 th group were kept as controls.

	The safety of iradiated vaccine was proved in vaccinated chicks within 4 weeks under complete observation. Measurement of the immune response of the attenuated vaccine in bothe vaccinated groups using ELISA technique, revealed high titers of anti- E-tenella antibodies.

	Post challenge all vaccinated chicks provoked a satisfactory immunity against local virulent strain of E.tenella. Vaccineated chicks (group A and B) showed no symptons of the diseases with lower number of oocysts output and no deaths. The second vaccinated group (group B) recorded best results than the frist( group A) Susceptible chicks (group D) showed caecal coccidiosis with bloody diarrhoea and some chicks died.