Egyptian .J. Virol. 1, 547-552 (2004)

PREPARATION AND EVALUATION OF ANTI-RABBIT IGY PEROXIDASE AND FITC CONJUGATES

N.A. SHERIF, LAMIAA M. OMAR, S.M. SHEFEI AND ELHAM A. EL-EBIARY

Central laboratory for control of veterinary biologics abbasia Cairo.

Summary
 


In this investigation, a trial for perparation of anti-rabbit immunoglobulin-Y (Igy) peroxidase and fluorescein isothocyanate (FITC) conjugates was conducted. Rabbit IgG was separated and injected into specific pathogen free (SPF) Laying hens, from which their eggs were collected.

 
 
 


The immunoglobuln-Y (igY) was precipitated from the egg ylok of immunized hens by 33% saturated ammonium sulphate solution.
The anti-rabbit immunoglobulin-Y (IsgY) was purified by using affinity chromatograply. The obtaind globulins were divided into 2 parts.

 
 
 


The 1 st one was conjugated with preoxidase and the 2 nd part was conjugated with fluorescein isothiocyanate. Both conjugates gave a strong positive result when diluted 1:2500 and 1:50 respectively. The results indicated thay could be used successfully for detection of antigens and antibodies.