..::Veterinary Serum and Vaccine Research Institute::..

Beni - Suef Vet. J. Vol. XI. No. (2) Oct. 2001 : (237-246).

Preparation of Combined Vaccine against Canine distemper and Rabies

Edries, S.M.; Guirguis, W.I.; Khodeir, M.H. and Attyat, M. Kotb

Veterinary Serum and Vaccine Research Institute, Abbassia – Cairo.

Summary

	An inactivated cell culture combined vaccine was prepared against canine distemper and rabies. ERA strain of rabies virus propagated on BBHK-21 and canine distemper virus propagated on Vero cells were inactivated by binary ethyleneimine ( BEI) at 37 o C. The inactivation time was 4 hours for rabies and 6 hours for canine distemper. The two viruses were inactvated simultaneously for 6 hours.

	As the protective dose of each vaccine was previously deterimined, the total virus protein was estimated in such doses and accordingly the two inactivated viruses were mixed together and alhydragel was added as adjuvant.

	Different groups of puppies ( 3-5 months of age) were vaccinated with single rabies inactivated vaccine, single canine distemper inactivated vaccine.and the prepared combined rabies and canine distemper vaccine. Serological tests showed that all vaccinated animals exhibited good levels of specific antibodies against rabies and canine distemper without any antagonizing effect on their immune response in case of using combined vaccine. Also, the combined vaccine was found to be safe and immunogenic.