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| Egyption J. Virol.1, 547- 552 (2004)
Preparation and Evaluation of ANTI-rabbit IgY Peroxidase and FITC Conjugates
N. A. Sherif, Lamiaa M. Omar, S.M.Shafei and Elham A. El-Ebiary
Central Laboratory for Control of Veterinary Biologics, Abbassia, Cairo.
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| Summary |
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In this investigation, a trial for prepation of anti-rabit immunoglobulin-Y(IgY) peroxidase and fluorescsin isothiocyanate (FITC) conjugates was conducted Rabit IgG was separated and injected into specific pathogen free (SPF)laying hens.from which their eggs were collected. The immunoglobulin-Y(IgY) was precipitated from the egg yolk of immunized hens by 33% saturated sulphate solution.
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The anti-rabbit immunoglobulin-Y (IgG) was purified by using affinity chromatography. The obtained globulins were divided into 2 pars.
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The 1 st one was conjugated with peroxidase and the 2 nd part was conjugated with fluorescein isothiocyanate Both conjugates gave a strong positive result when diluted 1:2500 and 1:50. respectively .The results indicated that they could be used successfully for detection of antigens and antibodies.
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