..::Veterinary Serum and Vaccine Research Institute::..

Vet. Med. J. Giza. Vol 52, No. 3. (2004) : 335-346

Trials for preparation and evaluation of peroxidase and fluorescent isothiocyanate
conjugated antisera against equine influenza virus

M.M ., El-Kabbany and F.F. Zaki

Veterinary Serum and Vaccine Research Institute, Central Laboratory for Evaluation
of Veterinary Biologics Abbassia, Cairo , Egypt.

Summary

	The present study was designed to produce anti-influenza antibodies conjugated by peroxidase and fluorescein isothiocyanate for rapid detection of equine influenza (Ei) virus five rabbits were immunized with equine influenza (Ei) virus (A/ equi/2) and one was kept as control immunoglobulins were separated using concentrated ammonium sulphate.

	The globulin content was quanitated and it was 2.5 gm/ dl. These globulins were divided into 2 portions, the 1 st one was conjugated with peroxides and gave a strong positive result when diluted 1: 2000.

	The 2 nd portion was conjugated with strong positive result when diluted 1: 20 Both conjugates were tested and compared with an imported one on allantoic fluids previosuly proved to be positive to equine influenza and a strong positive reaction. The two prepared labeled antibodies could be used succesfully in detection of influenza antigen during the course of the discase.