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| J. Egypt. Vet.Med. Ass. 61,No 68:81-90 (2001)
Detection of Foot and Mouth Disease Virus using specific and
Non - Specifiv Fluorescent Antibody Conjugates
Abdel Aty, M.M. Fawzy, H.G.I., Fatma, A.A. Moussa and Saad, M.
Veterinary Serum and Vaccine Research Institute,
Abbassia, Cairo , Egypt.
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Detection of FMD virus from infected samples by fluorescent antibody technique using in comparison with two different conjugates which are anti-FMD serum conjugated with fluorescent isothiocyanate (anti-FMD FA conjugate) and staphylococcal protein A conjugated also with fluorescent isothiocyanate (SPA-FA conjugate).
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The two conjugates were prepared and evaluated. The working end point dilution of the conjugates were 1/20 and 1/15 for anti- FMD FA conjugate and SPA-FA conjugate, respectively.
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The results revealed that there is no difference between the two conjugates and it considered as closely similar. Also, it could be concluded that the use of SPA-FA conjugate is sensitive, cheap and non- species specific for detection of FMD virus when using direct and in direct FA techniques.
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