..::Veterinary Serum and Vaccine Research Institute::..

J. Egypt. Vet.Med. Ass. 63,No.2 157-165 (2003)

Immune response of Cattle Vaccinated with Oil Adjuvant Alhydragel
FMD Vaccines Inculated Subcutaneously and Intramuscularly

Roshdy, O.H.; A. Moussa; Aggour A.M.A,; El-Shehawy, L. and Daoud, A.M.

Veterinary Serum and Vaccine Research Institute,
Abbassia, Cairo , Egypt.

Summary

	Twelve calves were divided into four group, each includes there calves. The first group and the seco d one were each vaccinated with the inactivated alhydragel foot and mouth disease (FMD) vaccine either sub- cutancously (S/C) or intramuscularly (I/M).

	The third and the fourth grpups were each vaccinated with oil vaccine (Montanide ISA25 vaccine) either sub-cuaneously (S/C) or intramuscularly (I/M. The immune response estimated by serum neutralization test and ELISA revealed that the antibody titres of the oil FMD vaccine which injected intramuscularly (I/M) was more earlier and higher at the first eight weeks post vaccination than that of oil FMD vaccinated subcutancously (S/C) and the titres persisted for 28 weeks post vaccination for (S/C or I/M inoculation ).

	Local reactions at the site of inoculation were not observed in cattle vaccinated I/M with oil vaccines. Alhydragel vaccine injected by I/M or S/C route showed no local reaction. The antibody titers of the alhydrogel vaccine were lower than oil vaccine and persisted for 16 weeks only.