..::Veterinary Serum and Vaccine Research Institute::..

MINUFYIA VET.J. VOL.2 NO .1APRIL. 2002.

Preparation of an Inacivated Vaccine aganist hard pad Disease
(Canine Dictemper) in cats from the local isolate

Guirguis , W.I.:Azab, A.M.H;: Khodeir, M.H. and Daoud ,A.M.

Veterinary Serum and Vaccine Research Institute, Abbassia.Cairo.

Summary

	The local isolate of canine distemper (Hard pad) virus isolated from a stray cat and designed as CD- Abbassia/ 2001, was propagated in vero cell culture and inactivated with Binary Ethylene Amine (BEA) where the Alhydragel was added as adjuvant .Different doses (1,2&3 ml ) of such vaccine were inoculated S/C in different groups of cats (local breed ) first in the form of single dose and second time in the form of 2doses with apart 2 weeks in between.

	Three weeks later, the challenge test revealed that the most suitable protective dose is 2ml-inoculated S/C followed by a booster dose after 2 weeks. Vaccination of cats with 2 dose resulted in a protection ratio of 100% while the use of one dose induced a 60% protection.

	Higher dose (3ml) showed the same protection ratio as 2ml while the lower dose (1ml)did not induce a permissible proection. Serum neutralization test indicated that vaccinated cats exhibited good levels of specific neutralizing antibodies reached up titer of 32. Also the quality control test revealed that the prepared vaccine was free from foreign contaminants, safe and immunogenic.