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| J.Egypt. Vet.Med.Assoc.65,no 4: 191- 202, (2005).
Adaptation and Propagation of the Egyptian Strain of Pigeon Pox on different types of cell cultures
Olfat, E.Nakhla; Mervat, M. Ali; Michael,A. and Soad, M.Soliman
Veterinary Serum & Vaccine Research Institute, Abbassia,Cairo .P.OB.131, Egypt.
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| Summary |
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A virulent Egyptian strain of pigeon pox virus.(PPV).Isolated from a local outbreak, was adapted and attenuated in tissue cutures. The virus was primarly purified and propagated in 9-11day – old embryonated chicken eggs for three successive passages.
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The virus was adapted on chicken embryo fibroblast (CEF) cells, clear cytopathic effect (CPE ) appeared by the 7 th passage. This virus was transfered for adaptation and attenuation in African green monkey kidney cells (VERO), clear CPE appeared by the 17 th passage. The passage was continued with recording the titre (in tissue culture and embryonated chicken eggs) and the pathogenicity (in susceptible squabs) every 5 passages up to 27 passage.
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The results revealed that the adapted PPV reached its maximum titre ( 10 5-4 TCID 50 /ml at the 27 th passage on VERO cells and 10 7.2 EID 50 /ml in embryonated chicken eggs for the same passage)and lost its pathogenicity after 27 serial passages in tissue culture cells. The growth pattern of PPV in tissue cultures was monitored from the 1 st up to 120 th hour post inoculation, beside the detection of intracytoplasmic inclusion bodies specific to pox viruse.
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