THE EGYPTIAN JOURNAL OF IMMUNOLOGY.VOL 7 (1), 2000: 97-103.

Evaluation of Relationships among ELISA, Dot ELISA and Agar Gel precipitation
tests in the detection of 3 CD Antigens of FMDV

M. Shawky; H.El- Watany, A. Samira El-Kilany and O.H.Roshdy

Veterinary Serum & Vaccine Research Institute, Abbassia, Cairo.
Summary
 


Dot ELISA was used for the detection of antibodies against protease and polymerase genes (3CD) antigens in animals infected with foot and mouth disease virus (FMDV).

 
 
 


Antigen antibody reactions were detected with an antibovine serum labeled with hoseradish peroxidase. Using 4-chloro-1-naphthol as a substrate, positive reactions appeared as blue dots and the results were compared with those of solid phase ELISA and agar gel precipitation.

 
 
 


Dot ELISAappeared to have a potential for rapid and economical field test in the diagnosis of FMDin infected animals.