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| J. Egypt Vet. Med . Ass. 61.no.6B: 81-90, (2001)
Detection of Foot and Mouth Disease Virus using specific and non- specific fluorescent Antibody Conjugates
Abdel Aty, M.M.; Fawzy, H.G.I.; Fatma, A.A. Moussa and Saad, M.
FMD Dept Veterinary Serum and Vaccine Research Institute,
Abbassia, Cairo , Egypt.
Animal Reproduction Research Institute Giza , Egypt.
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| Summary |
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Dection of FMD virus from infected samples, fluorescent antibody technique was used using in comparison with two different conjugates which are anti-FMD serum conjugated with fluorescent isothiocyanate ( anti-FMD FA conjugate and staphylococcal protein A conjugated also with fluorescent isothing ocyanate (SPA-Fa conjugate).
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The two conjugates were prepared and evaluated. The working end point dilution of the conjugate were 1/20 and 1/15 for anti-FMD-FA conjugate and SPA-FA conjugate, respectively. |
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The results revealed that there is no different between the two conjugate and considcred closely similar. Also, it could concluded that the use of SPA-FA conjugate is sensitive, cheap and non-species specific for detection of FMD virus when using direct and indirect FA techniques. |
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