..::Veterinary Serum and Vaccine Research Institute::..

Egypt. J.Agric. Res. 79 (4) , 2001.

Studies on the effect of different preservatives on the
quality control of Antitetanic Serum

Fayka Kamal 1 , S.M. Shafey 2 , M.M.Awad 1 and A. El-Sayed 1

Veterinary Serum and Vaccine Research Institute,Agricural Research Centre,
Ministry of Agricultur, Abbassia, Cairo, Egypt.

Summary

	The effects of different preservatives on the keeping quality of antitetanic serum were investigated. The following preservatives were added cresol 0.3% cresol 0.4% phenol 0.5% phenol 0.25% phenol0.5% and merthiolate 1:10.000, merthiolate 1:5000, sodum azide1:10.000, sodium azide 1:8000, sodum azide1:5000.

	Sterility test, total protein, physical appearance, pH values, safety and potency test were studied on each product after one year of storage. In this study, it is proved that the best quality was seen in the antitetanic serum with cresol 0.4% and with sodium azide 1:5000.Both products were clear in appearance, completely sterile from aerobic,anaerobic bactria, fungus and mycoplasma.

	They were potent and safe when injected in horses. while,antitetanic serum with phenol 0.5% although it was sterile, potent and safe for injection, it gave turbidity on storage.