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| Differntiation between Virulent and Avirulent Strains of Pasteurella
Multocida using Restriction Endonuclease Analysis
S.S. Salama,S.M. ShafeI, and Elham A.El- Ebiary
Central Laboratory for Evaluation of Veterinary Biologics, Abbassia,Cairo. |
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In this study we used one reference Climson University (CU) stain and 3 virulent field strains isolated from naturally infected chicken and identified as P.Multocida.
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DNA was extracted from all strains and subjected to restriction endonuclease analysis, using EcoRI, HpaIIand Hind III revealing that,great similarity between either the reference or local virulent field isolates.
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The obtained results indicated that the most differentiable restriction endonuclease enzyme was the Hind III, which showed differtent band patterns between different strains.
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